Measurement of microbial biomass N:C by chloroform fumigation-incubation

Soil Microbial Biomass Determination

Basic Idea: The quantity of microbial biomass in a given soil sample is inherently difficult to measure. To some degree, any measurement of microbial biomass is relative – different methodologies or variations in methodologies will yield microbial biomass estimates that are not directly comparable. For this reason, if you want to measure microbial biomass – choose a method and stick with it exa...

Responses of soil microeukaryotic communities to short-term fumigation-incubation revealed by MiSeq amplicon sequencing

In soil microbiology, there is a "paradox" of soil organic carbon (SOC) mineralization, which is that even though chloroform fumigation destroys majority of the soil microbial biomass, SOC mineralization continues at the same rate as in the non-fumigated soil during the incubation period. Soil microeukaryotes as important SOC decomposers, however, their community-level responses to chloroform f...

Estimation of Microbial Biomass Carbon and Nitrogen in Bangladesh Soils

In order to estimate the microbial biomass content and to quantify the amount of available plant nutrients derived from microbial biornass in Bangladesh soils, biomass-C, -N, and available-N eoptents using samples from 8 soils were measured by applying the chloroform fumigation and drying-rewetting methods, respectively. 1)

Uncertainties in determining microbial biomass C using the chloroform fumigation–extraction method

Soil CO2 evolution: Response from arginine additions

Short-term response of soil C mineralization following drying/rewetting has been proposed as an indicator of soil microbial activity. Houston Black clay was amended with four rates of arginine to vary microbial responses and keep other soil properties constant. The evolution of CO2 during 1 and 3 days following rewetting of dried soil was highly related to CO2 evolution during 10 days following...